Regulation of miR-181 on chemotherapy-resistant cervical cancer cells

MiR-181 was highly expressed in lung cancer cells of cisplatin-resistance. In this study, we sought to explore the connection of miR-181 expression with cell proliferation, invasion and apoptosis in cervical cancer cells. Real-time polymerase chain reaction and Western blot were performed to determine the expression level of miR181 at mRNA and protein level. Then MTT and flow cytometry were respectively used to explore the proliferation, apoptosis in cervical cancer cells, lactic dehydrogenase assay was performed to test the cytotoxicity. The results suggested that overexpression of miR-181 significantly increased the proliferation and decreased apoptosis of cytotoxicity cervical cancer cells compared with the control group. Moreover, LDH activity of miR-181-overexpressed cells was significantly decreased, suggesting reduction of the cytotoxicity and the increase of drugs-resistance. These results proved that miR-181 could be tightly involved in protection of cervical cancer. This study revealed the key roles of miR-181 and its potential in treating cervical cancer.